Development of a loopmediated isothermal amplification assay. The name trichosporon cutaneum is often used synonymously with trichosporon beigelii. Trichosporon asahii on cled agar after overnight incubation. Mixed infection of toe nail caused by trichosporon asahii and. Trichosporon asahii an overview sciencedirect topics. Trichosporon asahii was the most common species 273358, 76. The patient was treated with amphotericin b and fluconazole, but t. Trichosporon asahii, which is a ubiquitous and exclusively. The infection occurs in both the tropics and temperate zones. Trichosporon beigelii is a minor component of normal skin flora, and is widely distributed in nature, but nevertheless is the most significant pathogen in the genus trichosporon. Trichosporon asahii, a noncandida yeast that caused fatal.
Species distribution and antifungal susceptibility of 358. Rapid and simple detection of trichosporon asahii by. Download child taxa of trichosporon download species of trichosporon search for child taxa of trichosporon classification unranked biota kingdom fungi phylum basidiomycota subphylum agaricomycotina class tremellomycetes order trichosporonales family trichosporonaceae genus trichosporon species trichosporon asahii akagi ex sugita, a. Invasive trichosporon species have been documented mostly in neutropenic patients with underlying. Hay, in mandell, douglas, and bennetts principles and practice of infectious diseases eighth edition, 2015. Sep 24, 2014 trichosporon asahii is a rare but emerging fungal pathogen that causes severe and lifethreatening infections with high mortality rate, mostly in immunocompromised patients. Trichosporon asahii infection in a patient with metastatic. May 06, 2014 a fatal case of trichosporon asahii fungemia and pneumonia in a kidney transplant recipient during caspofungin treatment meifang yang,1,2 hainv gao,1,2 lanjuan li1,2 1state key laboratory for diagnosis and treatment of infectious diseases, the first affiliated hospital, school of medicine, zhejiang university. Proliferation of trichosporon yeasts in the hair can lead to an unpleasant but nonserious condition. In vitro activity of diphenyl diselenide and ebselen alone. Trichosporon pullulans phadia setting the standard. Clinical breakpoints have not been defined for trichosporon species, and the mics cannot be reliably interpreted as indicating resistance or susceptibility.
Pdf bloodstream infections due to trichosporon spp. Trichosporon asahii is the most commonly reported species. Trichosporon asahii among intensive care unit patients at a medical center in jamaica volume 34 issue 6 robyn neblett fanfair, orville heslop, kizee etienne, lois rainford, monika roy, lalitha gade, joyce peterson, heather oconnell, judith noblewang, s. Trichosporonosis definition of trichosporonosis by medical. The fungal keratitis was successfully treated with topical amphotericin b and oral ketoconazole. Januarymarch 2007 rastogi et al invasive trichosporonosis in nonimmunocompromised host trichosporonosis with trichosporon asahii. Trichosporon asahii an unusual cause of urinary tract.
During mycologic monitoring of the air in a hematology ward, we found massive air contamination caused by trichosporon asahii, both in the room where neutropenic patients were staying and the corridor immediately outside the room. Trichosporon definition of trichosporon by medical. In particular, the name trichosporon beigelii is now obsolete, and previously described infections reported in the literature under this name could in fact be due to any one of the species listed below. Hospital, bangalore with a diagnosis of tubercular meningitis and urinary tract infection. Successful treatment of trichosporon asahii fungemia with.
Trichosporon definition of trichosporon by medical dictionary. Cardiac infectionrobert grignani et al 439 an unusual infection in a child with congenital heart disease trichosporon asahii infection with rapid diagnosis by 18s ribonucleic acid rna. Bloodstream infections due to trichosporon spp species distribution, trichosporon asahii genotypes determined on the basis of ribosomal dna intergenic spacer 1. Trichosporon asahii was cultured from the patients ureteral stent. Until the end of the 20th century, a wide range of species were included under the name of trichosporon beigelii or synonyms, which were later shown to be phylogenetically distinct. Although important, little is known about the cohabitation of t. Contact lens wear is a known risk factor for fungal keratitis. The ability to form biofilms, which are composed of budding yeasts and filamentous forms embedded within an extracellular polysaccharide eps matrix, is thought colloquially by many medical and. However, hypersensitivity pneumonitis induced by 2 members of the trichosporon species, t. Detection and characterization of plasminogen receptors on. We detected the speciesspecific intergenic spacers igs of rrna gene of t. Using 1 h and cnmr spectra and electron ionizationhigh resolution mass spectrometry hrnegativefabms, we. Full text a fatal case of trichosporon asahii fungemia and.
A case report and an update a chowdhary, s ahmad, zu khan, dc doval, hs randhawa department of medical mycology, vallabhbhai patel chest institute, university of delhi, new delhi 110 007, india. Trichosporon asahii infection in an advanced aids patient. Trichosporon asahii is an opportunistic fungus that causes infections in immunosuppressed patients. Trichosporon asahii trichosporon beigelii infections are rare but have been associated with a wide spectrum of clinical manifestations, ranging from superficial involvement in immunocompetent individuals to severe systemic disease in immunocompromised patients. Trichosporon mycotoxinivorans, a novel respiratory pathogen in patients with cystic fibrosis. Onychomycosis prevalence rate ranges from 2 to 30%, which were 50% of nail diseases and 30% of superficial mycosis, respectively. Trichosporon asahii is well recognized as a pathogen causing summertype hypersensitivity pneumonitis in japan 1. Trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen seen particularly in. Trichosporon asahii infection after total knee arthroplasty. Trichosporon asahii sepsis in a patient with pediatric. Antifungal susceptibility testing for amphotericin b, fluconazole, voriconazole and posaconazole followed the clinical and laboratory standards institute reference method. Integrated transcriptomic analysis of trichosporon asahii. Trichosporon asahii is the major pathogen causing invasive trichosporonosis.
Pdf trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen seen particularly in immunologically. Biofilm formation leads to antifungal drug resistance, so biofilmrelated infections are relatively difficult to treat and infected devices often require surgical removal. Sami ulus maternity and children s health and diseases training and research hospital, department of pediatric oncology and haematology, ankara, turkey. The colonies are usually raised and have a waxy appearance, which develop radial furrows and irregular folds. This study evaluated the in vitro susceptibility of trichosporon asahii strains to diphenyl diselenide dpds and ebselen ebs alone and in combination with amphotericin b amb, fluconazole fcz, itraconazole itz and caspofungin cas using the microdilution method. Urinary tract infection by trichosporon asahii sood s, pathak. Nov 06, 2014 trichosporonosis is an emerging infection predominantly caused by trichosporon asahii which is a ubiquitous and exclusively anamorphic yeast. Case reportinvasive trichosporonosis due to trichosporon asahii. Designated 150 years ago, the genus trichosporon was for many years a collection of many different yeastlike organisms. A case urinary tract infection of trichosporon asahii in a patient. Nov 23, 2019 trichosporon asahii and rhodotorula mucilaginosa are important fungal species causing disseminated disease in immunocompromised patients. This study reports on a case of fungemia due to trichosporon asahii in a nonneutropenic patient with.
Pdf trichosporonosis is an emerging infection predominantly caused by trichosporon asahii which is a ubiquitous and exclusively anamorphic yeast. We noted a good evolution and disappearance of the casts after the. The patient was advised to keep the hair dry, and was treated with 2% ketoconazole shampoo topically daily for 2 months with oral fluconazole for 3 months. After 1 month, a creeping pellicle and sediment are formed. An unusual case of nosocomial trichosporon asahii fungemia in a. Reports of fungal infection after total knee arthroplasty are extremely rare. Jan 28, 20 shashidhar, v 2009 trichosporon asahii an unusual cause of urinary tract infection in an aids patient. A detailed interrogation revealed the history of tying wet hairs after washing. Trichosporon asahii is an emerging, lifethreatening opportunistic pathogen. By multivariate analysis, breakthrough infection or 2. Trichosporon is a genus of anamorphic fungi in the family trichosporonaceae. Sep 01, 2001 trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen seen particularly in immunologically compromised patients. Here we describe trichosporon species distribution and prevalence of. Trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen seen particularly in immunologically compromised patients.
Successful treatment of breakthrough disseminated trichosporon. Trichosporon asahii is a basidiomycetous yeast which causes white piedra and onychomycosis in. Trichosporon species are ureasepositive, nonencapsulated basidiomycetous yeasts characterised by the development of hyaline, septate hyphae that fragment into oval or rectangular arthroconidia. To the best of our knowledge this is the first report of a urinary tract infection caused by trichosporon asahii from western india. This study evaluated the in vitro susceptibility of trichosporon asahii strains to diphenyl diselenide dpds and ebselen ebs alone and in combination with amphotericin b amb, fluconazole fcz. Multidrugresistant trichosporon asahii infection of nongranulocytopenic patients in three intensive. We present two pediatric oncology cases successfully treated with voriconazole after t. A fatal outbreak of trichosporon asahii sepsis in a neonatal intensive care unit. Trichosporon asahii is not only the causative agent of trichosporonosis, but also causes shp representing type iii or iv allergy, which may develop after repeated inhalation of arthroconidia.
It could be easily misdiagnosed due to lack of awareness, especially when invasive or deepseated infections occur in nonimmunocompromised patients, and inadequately treated since the clinical failures and high minimum. Invasive trichosporonosis caused by trichosporon asahii and other unusual trichosporon species at a medical center in taiwan. This pdf is available for free download from a site hosted. Differentiation of the emerging human pathogens trichosporon. Most are typically isolated from soil, but several species occur as a natural part of the skin microbiota of humans and other animals. Two case presentations infected by trichosporon asahii and treated with voriconazole successfully hikmetgulsahtanyildiz,suleyesil,suletoprak,mehmetonurcandir,andgursessahin dr. In 1902 vuillemin grouped the species described for this genus under the trichosporon beigelii denomination, a practice that remained until the late 90s. There are now 100 reported cases of hematogenously disseminated infections with this lifethreatening yeast, and no effective antifungal therapy is available. Here we describe trichosporon species distribution and prevalence of trichosporon asahii genotypes based on rdna igs1 sequencing as well as antifungal. Infeccion sistemica por trichosporon asahii request pdf. White piedra is an uncommon infection caused by yeasts of the genus trichosporon, namely, trichosporon ovoides scalp hair, trichosporon inkin pubic hair, and trichosporon asahii rare in piedra.
Trichosporon asahii was the main causative species 46. A rare case of urinary tract infection due to trichosporon. In human they are found in the skin, gastrointestinal tract and respiratory tract. Fungemia due to trichosporon asahii in a neutropenic child refractory to amphotericin b. Sequencing of the ribosomal dna rdna intergenic spacer 1 igs1 is currently mandatory for accurate trichosporon identification, but it is not usually performed in routine laboratories. Trichosporon asahii is the most frequently isolated species in invasive trichosporonosis 2.
With the advent of molecular techniques, the genus was rearranged, and many of its species. The increase in profoundly immunocompromised patients has been accompanied by an increase not only in frequency of opportunistic infections, but also in the variety of species involved. The molecular weight of bg1 and bg2 were estimated as 160 kda and 30 kda, respectively. Trichosporon asahii infection with rapid diagnosis mafiadoc. Trichosporonosis definition of trichosporonosis by. Trichosporon asahii septic thrombophlebitis following lower. Therefore, prevention of biofilm formation is important in clinical settings. Trichosporon asahii trichosporon beigelii infections are rare but have been associated with a wide. Trichosporon asahii was the predominant species and corresponded to 43% of isolates. Trichosporon asahii urinary tract infection in immunocompetent patients. Trichosporon genus is subdivided into six distinct human pathogenic species of which t. We report the first known case of fungal keratitis caused by t. Trichosporon asahii keratitis in a patient with a type i. In most reports, the infecting organism is a candida species.
It is a yeastlike fungus, often found in soil, decomposing wood, air, rivers, lakes, seawater, cheese, scarab beetles, bird droppings, bats, pigeons and cattle. This pdf is available for free download from a site hosted by medknow publications. Trichosporon asahii as an emerging etiologic agent of disseminated trichosporonosis. A 38yearold hiv seropositive male patient was referred to bowring and l.
The most common serotype ii species isolated from the homes of shp patients is t. Conventional methods of its detection are timeconsuming or costly and often require complex dna extraction and purification steps, which hinders rapid clinical diagnosis. The journal of the academy of clinical microbiologists, 11 1. Two case presentations infected by trichosporon asahii and. Growth inhibition of an opportunistic yeast pathogen. All species of trichosporon are yeasts with no known teleomorphs sexual states. The other species of trichosporon did not show high mics of amphotericin b, and gm mics were trichosporon spp. A chowdhary, s ahmad, zu khan, dc doval, hs randhawa. Feb 12, 2018 a fatal outbreak of trichosporon asahii sepsis in a neonatal intensive care unit.
Trichosporon asahii among intensive care unit patients at. Bloodstream infections due to trichosporon spp species. Trichosporon asahii trichosporon beigelii infections are rare but have been associated with a wide spectrum of clinical manifestations, ranging from superficial involvement in immunocompetent individuals to severe systemic disease in immunocompromised. Facing the clinical and dermoscopic aspect of the lesions, the diagnosis of white piedra was suggested then confirmed by a mycological examination which revealed a trichosporon asahii. Neutropenia developing due to malignancies is an important risk factor for fungal infection. Trichosporon asahii was identified from blood cultures, confirmed by morphological examination on cornmeal agar and speciated by biochemical analysis api 20c. A 67 year old male presented with a widespread erythematous rash prior to sustaining a syncopal episode outside the dermatologistsof.
Mar 10, 2020 trichosporon asahii is the major causative fungus of disseminated or deepseated trichosporonosis and forms a biofilm on medical devices. The reevaluation of the genus trichosporon has led to the replacement of the old taxon trichosporon beigelii by six new species. In the coculture of staphylococcus epidermidis and trichosporon asahii, a fungal pathogen, it was observed that live s. Trichosporon asahii can be an opportunistic pathogen, causing invasive disease and sometimes disseminated infections, usually in immunocompromised patients. Trichosporon asteroides is an asexual basidiomycetous fungus first described from human skin but is now mainly isolated from blood and urine. We examined the ability of surface molecules to bind human plasminogen using clinical isolates of t. Trichosporonosis is an emerging infection predominantly caused by trichosporon asahii which is a ubiquitous and exclusively anamorphic yeast.
It was identified as trichosporon asahii using api id 32c. Successful treatment of trichosporon asahii fungemia with isavuconazole in a patient with hematologic malignancies guillaume feugray,1 daphne krzisch,2 marion dehais,1 romy razakandrainibe,3 gilles gargala1,3, loic favennec1,3, stephane lepretre,2 vincent camus,2 damien costa1,31department of parasitologymycology, rouen university hospital, rouen, france. The virulence factors of this yeast pathogen remain uncharacterized. Trichosporon asahii is a rare cause of disseminated fungal infection in immunocompromised patients, presenting a diagnostic and therapeutic challenge. Trichosporon asahii infection in a immunosupressed patient. Yeastlike organisms were isolated from urine samples on three consecutive days as pure cultures. Trichosporon spp an emerging fungal pathogen medicina. In this study, we evaluated colony pcr, which directly uses colonies or trace clinical samples as the template for amplification, for rapid. Antachopoulos c, papakonstantinou e, dotis j, bibashi e, tamiolaki m, koliouskas d. Wolf dg, falk r, hacham m, theelen b, boekhout t, scorzetti g, et al.
Identification of these species is important, both for epidemiological purposes and for therapeutic management, but conventional identification based on biochemical traits is hindered by the lack of updates to the species databases provided by the different commercial systems. Trichosporon asahii is the major causative fungus of disseminated or deepseated trichosporonosis and forms a biofilm on medical devices. A rare case of recurrent urinary tract infection due to. It was identified as trichosporon asahii using standard techniques. Ebs showed in vitro activity against t asahii strains with minimal inhibitory concentration mic ranged from 0.
Invasive trichosporonosis is a deep mycosis found mainly in immunocompromised hosts, and the major pathogen is trichosporon asahii. Infections by this species usually respond to treatment with azoles and amphotericin b. Pdf trichosporon asahii infection in a immunosupressed. Full text a fatal case of trichosporon asahii fungemia. Trichosporon asahii has primarily been isolated from blood, lung tissue and urine of patients suffering from deepseated trichosporonosis.
Multidrugresistant trichosporon asahii infection of. Hemorrhagic necrotizing pneumonia caused by trichosporon. A rare case of disseminated trichosporon asahii infection. Trichosporon inkin as an emergent pathogen in patients with. The patient was advised to keep the hair dry, and was treated with 2%.
Trichosporon trikosporon, trikosporon, a genus of imperfect fungi that possess branching septate hyphae with arthroconidia and blastoconidia. October 2006 sood et al urinary tract infection by t. Trichosporon is an uncommon agent of fungal keratitis. Prompt diagnosis and effective treatment of trichosporon. Download fulltext pdf download fulltext pdf trichosporon asahii urinary tract infection in immunocompetent patients article pdf available in medical journal armed forces india 714. We report a case of urinary tract infection due to trichosporon asahii in an 80yearold male patient with idiopathic dilated cardiomyopathy. Trichosporon asahii is the major causative agent of deep.